Metrohm 940 Professional IC Vario ONE/ChS/PP/HPG Bedienungsanleitung

940 Professional IC Vario940 Professional IC Vario ONE/ChS/PP/HPGManual8.940.8010EN

1.1 Instrument description￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)Leak sensorThe leak sensor detects leaki

5.13 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭92￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)5.13.2.2 Replacing the filterThe filters in

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭935.14 Servicing the detectorFollow the

5.16 Separation column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭94￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)Checking the rinsing timeYou can determine

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭955.16.2 Protecting the separation colu

5.17 Quality Management and qualification with Metrohm￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭96￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)5.17 Quali

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6 Troubleshooting940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭976 TroubleshootingProblem Cause RemedyMarked dro

6.1 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭98￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)Problem Cause RemedySeparation column –Blocked.￭ Regenerate t

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6 Troubleshooting940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭99Problem Cause RemedyIndividual peaks aregreater

6.1 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭100￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)Problem Cause RemedyChromatogramshave poor resolutionSeparat

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭7 Technical specifications940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭1017 Technical specifications7.1 Refere

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭3Peristaltic pumpThe peristaltic pump is used for pum

7.4 Housing￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭102￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)7.4 HousingDimensionsWidth 365 mmHeight 642 mmDepth

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭7 Technical specifications940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭1037.7 Eluent degasserMaterial Fluoropol

7.9 Injection valve￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭104￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)Gradient capabil-ityIsocratic or gradient (ex

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭7 Technical specifications940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭1057.12 DetectorYou can find the technic

7.16 Safety specification￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭106￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)7.16 Safety specificationThis instrume

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Warranty (guarantee)940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭1078 Warranty (guarantee)Metrohm guarantees

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭108￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)The same warranty periods that are specified for a corresponding

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭9 Accessories940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭1099 AccessoriesUp-to-date information on the scope o

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭110￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)The Partslists webpage will be displayed. 7Select the desired ou

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Index940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭111IndexAAmbient conditions ... 101Aspiration fil

1.3 Safety instructions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)1.3 Safety instructions1.3.1 General note

Index￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭112￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)LoopSee also "Sample loop" ... 28MMains volta

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭5Protection against electrostatic chargesWARNINGElect

1.4 Symbols and conventions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)More details about the disposal of your

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Overview of the instrument940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭72 Overview of the instrument2.1 Fron

2.1 Front￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)5Column thermostatWith two column holders for two separat

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Overview of the instrument940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭92.2 RearFigure 2 Rear1Bottle holderOf

2.3 Feed-throughs for capillaries and cables￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭10￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)5Vacuum connectionFor

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Overview of the instrument940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭11Openings on the doorFigure 3 Feed-thr

2.3 Feed-throughs for capillaries and cables￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭12￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)Openings on the back

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Overview of the instrument940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭13Figure 5 Ducts for capillaries

3.1 Setting up the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭14￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)3 Installation3.1 Setting up the i

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭15Also see: PEEK pressure screws video on the Interne

3.2 Capillary connections in the IC system￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭16￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭ The capillary ends do

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭173.3 Removing the handleThe instrument is equipped

3.4 Removing transport locking screws￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭18￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)3.4 Removing transport lock

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭19Store the transport locking screws in a safe place.

3.5 Connecting the drainage tubing and leak sensor￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭20￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)Connecting the

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭21Route the loose end into a waste container.3.5.2 C

Metrohm AGCH-9100 HerisauSwitzerlandPhone +41 71 353 85 85Fax +41 71 353 89 [email protected] Professional IC Vario940 Professional

3.7 Connecting the eluent bottle￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭22￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)3.7 Connecting the eluent bottle

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭23 2Mounting the tubing adapter and the aspiration fi

3.7 Connecting the eluent bottle￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭24￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340) 4Mounting the adsorber tubeNOTED

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭253.8 Connecting the eluent degasserThe high-pressur

3.10 Installing an inline filter￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭26￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)Figure 9 High-pressure pump with

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭27Figure 10 Inline filter1Input capillaryConnected to

3.12 Injection valve￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭28￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)3.12 Injection valveThe injection valve conn

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭29Figure 12 Exchanging the sample loop1Pressure screw

3.13 Suppressor￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭30￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)3.13 SuppressorThe suppressor drive of the 940 Pr

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭31CAUTIONThe rotor may be destroyed during start-up i

TeachwareMetrohm AGCH-9100 [email protected] documentation is protected by copyright. All rights reserved.Although all the information

3.13 Suppressor￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭32￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)NOTEThe rotor's sealing surface is approximat

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭33￭ Insert the rotor into the adapter so that the tub

3.13 Suppressor￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭34￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)Recommended installationAlternative installationIn

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭353.13.2.1 Installing the eluent pathThe eluent path

3.13 Suppressor￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭36￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340) 1Prepare a tubing cartridge of the peristaltic pu

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭37Connecting the rinsing solution inlet to the perist

3.14 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭38￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)Order num-berName Material Inner diame-terUs

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭39Pump tubing Adapter6.1826.390 (yellow/yellow)6.1826

3.14 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭40￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)Installing the pump tubingFor this step you

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭41￭ Install the pump tubing connection with safety de

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Table of contents940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭IIITable of contents1 Introduction 11.1 Instrume

3.14 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭42￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)Input Tighten the aspiration tubing (6.1803.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭43Setting the contact pressure correctly 1￭ Fully loo

3.15 Installing the detector￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭44￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)Figure 14 Peristaltic pump1Contact pr

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭45 1Connect the out capillary of the suppressor and t

3.16 Connecting the sample degasser (optional)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭46￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)Connecting the samp

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭473.17 Installing the high-pressure gradient moduleT

3.19 Connecting the instrument to the power supply￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭48￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)Connecting the

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭49Connecting the power supply cable 1Inserting the po

3.20 Initial start-up￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭50￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)Rinsing the IC systemCAUTIONThe guard column

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭51 4Deaerating the high-pressure pump￭ Push the end o

Table of contents￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭IV￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)3.13.2 Connecting the suppressor ...

3.21 Connecting and rinsing the guard column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭52￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)3.21 Connecting and

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭53￭ Guard column (suitable for the separation column)

3.22 Connecting the separation column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭54￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭ Start manual control in Ma

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭55NOTEThe separation column may not be connected unti

3.22 Connecting the separation column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭56￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)Connecting the separation co

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭57 5Installing the output of the separation column￭ F

3.23 Conditioning￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭58￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭ Ensure that the eluent aspiration tubing is im

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭594 OperationThe 940 Professional IC Vario – ONE/ChS/PP

5.1 IC system￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭60￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)5 Operation and maintenance5.1 IC system5.1.1 Ope

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭615.1.4 Shutting down and starting back

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Table of contents940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭V5.13.2 Servicing the peristaltic pump ...

5.2 Capillary connections￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭62￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)5.2 Capillary connectionsAll connection

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭6313211112Figure 15 Column thermostat1Re

5.5 Handling the eluent￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭64￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)Replacing the preheating capillariesIn ord

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭65The composition of the eluent plays a

5.7 Notes on operating the high-pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭66￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)Connecting the conne

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭675.8 Servicing the high-pressure pumpF

5.8 Servicing the high-pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭68￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)CAUTIONMaintenance work on th

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭69Cleaning the outlet valve 1Removing th

5.8 Servicing the high-pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭70￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)NOTEThe maximum treatment tim

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭71Spare parts If the inlet valve cannot

Table of figures￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭VI￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)Table of figuresFigure 1 Front ...

5.8 Servicing the high-pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭72￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)NOTEThe maximum treatment tim

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭73￭ Has the pressure dissipated?￭ Is the

5.8 Servicing the high-pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭74￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)Figure 17 High-pressure pump

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭7521Figure 18 Tool for piston seal (6.26

5.8 Servicing the high-pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭76￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340) 3Removing the old piston sea

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭77Optional: Cleaning/replacing the zirco

5.8 Servicing the high-pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭78￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)Figure 21 Components of the p

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭79You can find these parts in the access

5.9 Servicing the inline filter￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭80￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)Connecting the input for the high-

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭81Removing the filterFigure 22 Inline fi

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭11 Introduction1.1 Instrument descriptionThe 940 Pr

5.9 Servicing the inline filter￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭82￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)Inserting a new filter 1Inserting

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭835.10 Servicing the pulsation absorber

5.12 Suppressor￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭84￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)CAUTIONThe suppressor has to be regenerated (see C

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭855.12.3.2 Regenerating the suppression

5.12 Suppressor￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭86￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭ In the software, use the Step command to switch

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭87 2Dismantling the suppressor￭ Unscrew

5.12 Suppressor￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭88￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭ Insert the rotor (23-3) into the housing (23-4)

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭89Replacing parts of the suppressor 1Dis

5.12 Suppressor￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭90￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭ The rotor's sealing surface is approximatel

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/ChS/PP/HPG (2.940.1340)￭￭￭￭￭￭￭￭915.13 Peristaltic pump5.13.1 Notes on