Metrohm 850 Professional IC Anion Bedienungsanleitung

850 Professional ICAnion – 2.850.2010Manual8.850.8045EN

4.12 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭90￭￭￭￭￭￭￭￭850 Professional IC – AnionOrdernumberName Material InnerdiameterUse6.1826.330 Pump tubing LFL(or

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – Anion￭￭￭￭￭￭￭￭91 3Mounting filter screw￭ Screw the filter screw (45-3) back into

4.14 Separation column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭92￭￭￭￭￭￭￭￭850 Professional IC – Anionspecial IC applications in the corresponding "Application Bullet

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – Anion￭￭￭￭￭￭￭￭934.15 Quality Management and validation with MetrohmQuality Mana

5.1 Problems and their solutions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭94￭￭￭￭￭￭￭￭850 Professional IC – Anion5 Troubleshooting5.1 Problems and their solutionsProblem

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Troubleshooting850 Professional IC – Anion￭￭￭￭￭￭￭￭95Problem Cause RemedyEluent – Evaporation of theorganic solvent in the elu-

5.1 Problems and their solutions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭96￭￭￭￭￭￭￭￭850 Professional IC – AnionProblem Cause RemedyMSM – regeneration orrinsing solution f

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Troubleshooting850 Professional IC – Anion￭￭￭￭￭￭￭￭97Problem Cause RemedyExtreme spread ofthe peaks in thechromatogram.Splittin

5.1 Problems and their solutions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭98￭￭￭￭￭￭￭￭850 Professional IC – AnionProblem Cause Remedy￭ Connect an FEP tubing between the ICi

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6 Technical specifications850 Professional IC – Anion￭￭￭￭￭￭￭￭996 Technical specifications6.1 Reference conditionsThe technical

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction850 Professional IC – Anion￭￭￭￭￭￭￭￭11 Introduction1.1 Instrument descriptionThe instrument 850 Professional IC –

6.5 Housing￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭100￭￭￭￭￭￭￭￭850 Professional IC – Anion6.5 HousingDimensionsWidth 365 mmHeight 642 mmDepth 380 mmMaterial of basetray

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6 Technical specifications850 Professional IC – Anion￭￭￭￭￭￭￭￭101Pressure rangePump 0…50.0 MPa (0…500 bar)Pump head 0…35.0 MPa (0

6.10 Column thermostat￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭102￭￭￭￭￭￭￭￭850 Professional IC – Anion6.10 Column thermostatType Peltier technique thermostat for two int

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6 Technical specifications850 Professional IC – Anion￭￭￭￭￭￭￭￭1036.13 Conductivity measuring systemType ￭ Microprocessor-control

6.14 Mains connection￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭104￭￭￭￭￭￭￭￭850 Professional IC – Anion6.14 Mains connectionRequired voltage 100…240 V ± 10 % (auto-sensing

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6 Technical specifications850 Professional IC – Anion￭￭￭￭￭￭￭￭1056.16 Safety specificationDesign / Test ￭ EN/IEC/UL 61010-1￭ CSA

7.1 Declaration of Conformity￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭106￭￭￭￭￭￭￭￭850 Professional IC – Anion7 Conformity and warranty7.1 Declaration of ConformityThis

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭7 Conformity and warranty850 Professional IC – Anion￭￭￭￭￭￭￭￭107EN 61010-1 Safety requirements for electrical equipment formeasur

7.3 Warranty (guarantee)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭108￭￭￭￭￭￭￭￭850 Professional IC – AnionSoftware developmentSoftware development occurs in terms of the so

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭7 Conformity and warranty850 Professional IC – Anion￭￭￭￭￭￭￭￭109Faults arising from circumstances that are not the responsibility

1.1 Instrument description￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2￭￭￭￭￭￭￭￭850 Professional IC – Anion1016827345911Figure 1 Front view of the instrument1Conductivity de

8.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭110￭￭￭￭￭￭￭￭850 Professional IC – Anion8 AccessoriesCautionÄnderungen vorbehalten!8.1 Scope of delivery 2.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Accessories850 Professional IC – Anion￭￭￭￭￭￭￭￭111Qty. Order no. Description1 6.1608.070 Eluent bottle / 2 L / GL 45Material: C

8.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭112￭￭￭￭￭￭￭￭850 Professional IC – AnionQty. Order no. Description1 6.1807.010 Y-connector for tubing 6-9 mm

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Accessories850 Professional IC – Anion￭￭￭￭￭￭￭￭113Qty. Order no. Description1 6.2023.020 Clip for SGJ 14/15Clip for SGJ 14/16Ma

8.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭114￭￭￭￭￭￭￭￭850 Professional IC – AnionQty. Order no. Description1 6.2621.030 Hexagon key 4 mmLength (mm): 7

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Accessories850 Professional IC – Anion￭￭￭￭￭￭￭￭115Qty. Order no. Description1 6.2626.000 Drain nozzle (ProfIC)Drain nozzle for

8.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭116￭￭￭￭￭￭￭￭850 Professional IC – AnionQty. Order no. Description2 6.2744.020 Luer/UNF couplingFor IC instru

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Accessories850 Professional IC – Anion￭￭￭￭￭￭￭￭117Qty. Order no. Description3 6.2744.070 Pressure screw shortShort version. Wit

8.1 Scope of delivery￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭118￭￭￭￭￭￭￭￭850 Professional IC – AnionQty. Order no. Description1 6.2816.020 Syringe 10 mL with Luer connec

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Accessories850 Professional IC – Anion￭￭￭￭￭￭￭￭119Qty. Order no. Description8.850.8045EN Manual for 850 Professional IC, 2.850.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction850 Professional IC – Anion￭￭￭￭￭￭￭￭3Eluent degasserThe eluent degasser removes gas bubbles and dissolved gases fro

8.2 Optional accessories￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭120￭￭￭￭￭￭￭￭850 Professional IC – AnionOrder no. Description6.1826.340 Pump tubing LFL (black/black), 3-s

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Accessories850 Professional IC – Anion￭￭￭￭￭￭￭￭121Order no. Description6.2057.090 Column holderFor holding separation columns i

8.2 Optional accessories￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭122￭￭￭￭￭￭￭￭850 Professional IC – AnionOrder no. Description6.6059.003 MagIC Net™ Multi – 10 additional l

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Index850 Professional IC – Anion￭￭￭￭￭￭￭￭123IndexNumbers/Symbols6.2821.090 aspiration filter ... 676.2821.130 filter ...

Index￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭124￭￭￭￭￭￭￭￭850 Professional IC – AnionImpurity MSMOrganic ... 83Initial installation ...

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Index850 Professional IC – Anion￭￭￭￭￭￭￭￭125Protection ... 3, 38, 92Regeneration ... 92Rinsing ..

1.2 Intended use￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4￭￭￭￭￭￭￭￭850 Professional IC – AnionConductivity detectorThe conductivity detector continuously measures the con

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction850 Professional IC – Anion￭￭￭￭￭￭￭￭51.3.1 Symbols and conventionsThe following symbols and styles are used in thi

1.4 Safety instructions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6￭￭￭￭￭￭￭￭850 Professional IC – Anion1.4 Safety instructions1.4.1 General notes on safetyWarningThis ins

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction850 Professional IC – Anion￭￭￭￭￭￭￭￭7Protection against electrostatic chargesWarningElectronic components are sensi

1.4 Safety instructions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8￭￭￭￭￭￭￭￭850 Professional IC – AnionMore details about the disposal of your old equipment can be obtained

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭92 Installation2.1 About this chapterThe Installation chapter contains￭ a lis

2.3 Flow diagram￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭10￭￭￭￭￭￭￭￭850 Professional IC – Anion 8Install sample path connections.￭ Connect the sample degasser (if require

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭11EluentSampleRegeneration solutionRinsing solutionSample optional 103245671381

2.4 Setting up the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭12￭￭￭￭￭￭￭￭850 Professional IC – Anion3High pressure pumpSee Chapter 2.10.4Purge valveSee Chapter 2.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭13The instrument should be protected against excessive temperature fluctu-ation

2.5 Capillary connections in the IC system￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭14￭￭￭￭￭￭￭￭850 Professional IC – AnionConnection capillaryPEEK capillaries and PTFE cap

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭152.6 Rear of the instrument2.6.1 Rollers and handleIn order to make transpor

2.6 Rear of the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭16￭￭￭￭￭￭￭￭850 Professional IC – AnionRemoving rollersProceed as follows to remove the rollers: 1Remove

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭17211Figure 5 Handle as MPak holder1Knurled screwsFor fastening the handle (5-2

2.6 Rear of the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭18￭￭￭￭￭￭￭￭850 Professional IC – Anion2.6.2 Positioning and connecting the detectorDetector 1Detector

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭19NoteUp to two detectors can be positioned and connected.CautionThe instrument

Metrohm AGCH-9101 HerisauSwitzerlandPhone +41 71 353 85 85Fax +41 71 353 89 [email protected] Professional ICAnion – 2.850.2010Manu

2.6 Rear of the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭20￭￭￭￭￭￭￭￭850 Professional IC – Anion￭ Connect the detector cable to the detector connection socketDet

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭21123Figure 7 Connection for the leak sensor on the rear of the instrument1Leak

2.6 Rear of the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭22￭￭￭￭￭￭￭￭850 Professional IC – Anion245613798Figure 8 Drainage tubing1Drainage tubing connectionFor d

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭237Drainage tubingSection of the 6.1816.020 silicon tubing.Guides escaped fluid

2.7 Capillary and cable feed-throughs￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭24￭￭￭￭￭￭￭￭850 Professional IC – Anion212134Figure 9 Capillary feed-throughs on the doors1Lu

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭251234567 88 76 59101112 1211109FrontRearFigure 10 Capillary feed-throughs base

2.8 Eluent￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭26￭￭￭￭￭￭￭￭850 Professional IC – Anion2.8 Eluent2.8.1 Connecting eluent bottleThe eluent is aspirated out of the elue

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭271 234Figure 11 Installing eluent bottle attachment1Eluent aspiration tubing 6

2.8 Eluent￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭28￭￭￭￭￭￭￭￭850 Professional IC – Anion 4Installing tubing weighting and aspiration filter1 2345Figure 13 Installing tub

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭29￭ Fasten the bottle attachment (13-2) on the eluent bottle (15-10).The aspira

TeachwareMetrohm AGCH-9101 [email protected] documentation is protected by copyright. All rights reserved.Although all the information

2.9 Eluent degasser￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭30￭￭￭￭￭￭￭￭850 Professional IC – Anion5Eluent6Aspiration filter 6.2821.0907Filter holderFrom accessory set 6.2

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭313Tubing flareWith tubing nipple.4Clamping screw5Eluent aspiration tubing 6.18

2.10 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭32￭￭￭￭￭￭￭￭850 Professional IC – Anion10891234562722211212213Figure 17 Capillary connections high pressure

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭33NoteThe eluent aspiration tubing is already installed in the newly deliveredi

2.10 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭34￭￭￭￭￭￭￭￭850 Professional IC – Anion2.10.2 Deaerating the high pressure pumpThe high pressure pump will

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭35Deaerating the high pressure pumpThe instrument must be connected to the PC a

2.11 Inline filter￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭36￭￭￭￭￭￭￭￭850 Professional IC – Anion 4Setting the flow rate￭ Start MagIC Net (if not yet started).￭ Ensure th

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭37Installing the inline filterCautionObserve the flow direction marked on the f

2.12 Pulsation damper￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭38￭￭￭￭￭￭￭￭850 Professional IC – AnionThe pulsation damper protects the separation column from damagecaused

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭392.13 Sample degasserThe sample degasser removes gas bubbles and disolved gas

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Table of contents850 Professional IC – Anion￭￭￭￭￭￭￭￭IIITable of contents1 Introduction 11.1 Instrument description ...

2.14 Injection valve￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭40￭￭￭￭￭￭￭￭850 Professional IC – Anion 4Guide the other end of the connection capillary out of the instrument

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭413Connection capillaryConnected to connector 4. Carries eluent tothe injection

2.14 Injection valve￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭42￭￭￭￭￭￭￭￭850 Professional IC – Anion124 53 6124 53 6124354312A BFigure 24 Injection valve – PositionsAPosit

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭432.15 Column thermostatThe column thermostat controls the temperature of the

2.15 Column thermostat￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭44￭￭￭￭￭￭￭￭850 Professional IC – Anion13211112Figure 25 Column thermostat1Capillary feed-throughsFor guidin

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭45NoteThe column input capillary is already threaded into the capillary recesse

2.16 Metrohm Suppressor Module (MSM)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭46￭￭￭￭￭￭￭￭850 Professional IC – Anion2.16 Metrohm Suppressor Module (MSM)2.16.1 General in

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭4713234567821Figure 26 MSM – connections1Union nut2MSM connecting piece 6.2832.

2.16 Metrohm Suppressor Module (MSM)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭48￭￭￭￭￭￭￭￭850 Professional IC – AnionConnecting the capillaries of the MSM 1Connecting the e

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭492.17 Peristaltic pump2.17.1 Principle of the peristaltic pumpThe Peristalti

Table of contents￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭IV￭￭￭￭￭￭￭￭850 Professional IC – Anion2.14 Injection valve ...

2.17 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭50￭￭￭￭￭￭￭￭850 Professional IC – Anion2.17.2 Installing the peristaltic pump6.2744.16016.2744.1802 3 4 5 6

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭51 3Connecting the pressure sideNoteDepending on the use of the peristaltic pum

2.17 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭52￭￭￭￭￭￭￭￭850 Professional IC – Anion123Figure 30 Install pump tubing connection without filter1Union nut2A

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭53Pump tubing Adapter6.1826.340 (black/black)6.1826.360 (white/white)6.1826.380

2.18 Conductivity detector￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭54￭￭￭￭￭￭￭￭850 Professional IC – Anion2.18 Conductivity detectorThe conductivity detector continuously

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭55132Figure 32 Conductivity detector rear1Detector cableWith mounted plug.2Dete

2.19 Connecting the instrument￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭56￭￭￭￭￭￭￭￭850 Professional IC – Anion1 234Figure 33 Connection detector – MSM1Detector input capil

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭571Connecting the mains cable￭ Plug the mains cable into the mains connection s

2.20 Guard column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭58￭￭￭￭￭￭￭￭850 Professional IC – AnionNoteThe guard column may only be installed after the initial start-up (see

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Installation850 Professional IC – Anion￭￭￭￭￭￭￭￭592.21 Separation columnThe intelligent separation column (iColumn) is the hea

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Table of contents850 Professional IC – Anion￭￭￭￭￭￭￭￭V4.9 Sample degasser ...

2.21 Separation column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭60￭￭￭￭￭￭￭￭850 Professional IC – AnionConnecting and rinsing the separation column 1Connecting the separati

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Start-up850 Professional IC – Anion￭￭￭￭￭￭￭￭613 Start-upThe chapter Start-up is divided into 2 sections:Initial start-up The i

3.2 Conditioning￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭62￭￭￭￭￭￭￭￭850 Professional IC – Anion 4Setting the contact pressure of the peristaltic pumpNoteThis work step ne

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Start-up850 Professional IC – Anion￭￭￭￭￭￭￭￭63 2Preparing the instrument￭ Ensure that the column is correctly mounted in accord

4.1 General information￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭64￭￭￭￭￭￭￭￭850 Professional IC – Anion4 Operation and maintenance4.1 General information4.1.1 CareWarni

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – Anion￭￭￭￭￭￭￭￭654.1.3 OperationCautionIn order to avoid disturbing temperature

4.3 Door￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭66￭￭￭￭￭￭￭￭850 Professional IC – Anion4.3 DoorCautionThe door is made of PMMA (polymethylmetacrylate). It must never bec

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – Anion￭￭￭￭￭￭￭￭674.4.2 Operation4.4.2.1 Supply bottleThe supply bottle with the

4.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭68￭￭￭￭￭￭￭￭850 Professional IC – AnionCautionIn order to spare the pump seals, the pump should not be opera

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – Anion￭￭￭￭￭￭￭￭69Cleaning/replacing the zirconium oxide pistonClean one piston af

Table of contents￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭VI￭￭￭￭￭￭￭￭850 Professional IC – Anion6.16 Safety specification ...

4.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭70￭￭￭￭￭￭￭￭850 Professional IC – Anion￭ Remove the backup ring from the pump head and lay to the otherparts

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – Anion￭￭￭￭￭￭￭￭71parts: a tip for removing the old piston seal and a sleeve for i

4.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭72￭￭￭￭￭￭￭￭850 Professional IC – Anion12Figure 37 Removing the piston seal1Piston seal2Tool for piston seal

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – Anion￭￭￭￭￭￭￭￭7312Figure 39 Inserting the piston seal into the pump head 4Replac

4.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭74￭￭￭￭￭￭￭￭850 Professional IC – Anion1234Figure 40 Removing valves1Outlet valve holder2Outlet valveOrder n

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – Anion￭￭￭￭￭￭￭￭75￭ Place the valve with the seal faced downwards above the recess

4.5 High pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭76￭￭￭￭￭￭￭￭850 Professional IC – Anion123567891098710546Figure 42 Components of the inlet valve and outlet

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – Anion￭￭￭￭￭￭￭￭77￭ Place the valve housing over the stacked components and hold i

4.6 Inline filter￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭78￭￭￭￭￭￭￭￭850 Professional IC – AnionMounting the pump headNoteTo prevent the pump head from being positioned t

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – Anion￭￭￭￭￭￭￭￭79Changing the filterThe flow must be stopped before changing the

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Table of figures850 Professional IC – Anion￭￭￭￭￭￭￭￭VIITable of figuresFigure 1 Front view of the instrument ...

4.7 Inline sample preparation￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭80￭￭￭￭￭￭￭￭850 Professional IC – Anion4.7 Inline sample preparationTo protect the separation column

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – Anion￭￭￭￭￭￭￭￭81 2Aspirating the sample and measuring timeAspirate a sample typi

4.9 Sample degasser￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭82￭￭￭￭￭￭￭￭850 Professional IC – Anion4.9 Sample degasser4.9.1 OperationIf you are working with sample degas

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – Anion￭￭￭￭￭￭￭￭83In order to record every new chromatogram under comparable condi

4.11 Metrohm Suppressor Module (MSM)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭84￭￭￭￭￭￭￭￭850 Professional IC – AnionNoteThe high pressure pump can be used for regeneration

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – Anion￭￭￭￭￭￭￭￭85123 45Figure 44 MSM – Components1Union nut2MSM connecting piece

4.11 Metrohm Suppressor Module (MSM)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭86￭￭￭￭￭￭￭￭850 Professional IC – Anion￭ Check whether solution emerges at the MSM connecting

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – Anion￭￭￭￭￭￭￭￭874.11.3.3 Replacing parts of the MSMIt may be necessary to repla

4.12 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭88￭￭￭￭￭￭￭￭850 Professional IC – Anion￭ Insert the new MSM rotor A (44-3) in the MSM housing (44-4) insuch a

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4 Operation and maintenance850 Professional IC – Anion￭￭￭￭￭￭￭￭89CautionThe 6.1826.xxx pump tubings consist of PVC or PP and ther